diff --git a/Lib/test/test_sqlite3/test_regression.py b/Lib/test/test_sqlite3/test_regression.py
index e00e1bc64c..44cfa8aa14 100644
--- a/Lib/test/test_sqlite3/test_regression.py
+++ b/Lib/test/test_sqlite3/test_regression.py
@@ -409,8 +409,6 @@ def callback(*args):
         del ref
         support.gc_collect()
 
-    # TODO: RUSTPYTHON
-    @unittest.expectedFailure
     def test_del_isolation_level_segfault(self):
         with self.assertRaises(AttributeError):
             del self.con.isolation_level
diff --git a/stdlib/src/sqlite.rs b/stdlib/src/sqlite.rs
index 744da0cf6e..ce70a5883d 100644
--- a/stdlib/src/sqlite.rs
+++ b/stdlib/src/sqlite.rs
@@ -61,7 +61,10 @@ mod _sqlite {
             PyInt, PyIntRef, PySlice, PyStr, PyStrRef, PyTuple, PyTupleRef, PyType, PyTypeRef,
         },
         convert::IntoObject,
-        function::{ArgCallable, ArgIterable, FsPath, FuncArgs, OptionalArg, PyComparisonValue},
+        function::{
+            ArgCallable, ArgIterable, FsPath, FuncArgs, OptionalArg, PyComparisonValue,
+            PySetterValue,
+        },
         object::{Traverse, TraverseFn},
         protocol::{PyBuffer, PyIterReturn, PyMappingMethods, PySequence, PySequenceMethods},
         sliceable::{SaturatedSliceIter, SliceableSequenceOp},
@@ -1357,22 +1360,32 @@ mod _sqlite {
             self.isolation_level.deref().map(|x| x.to_owned())
         }
         #[pygetset(setter)]
-        fn set_isolation_level(&self, val: Option<PyStrRef>, vm: &VirtualMachine) -> PyResult<()> {
-            if let Some(val) = &val {
-                begin_statement_ptr_from_isolation_level(val, vm)?;
-            }
+        fn set_isolation_level(
+            &self,
+            value: PySetterValue<Option<PyStrRef>>,
+            vm: &VirtualMachine,
+        ) -> PyResult<()> {
+            match value {
+                PySetterValue::Assign(value) => {
+                    if let Some(val_str) = &value {
+                        begin_statement_ptr_from_isolation_level(val_str, vm)?;
+                    }
 
-            // If setting isolation_level to None (auto-commit mode), commit any pending transaction
-            if val.is_none() {
-                let db = self.db_lock(vm)?;
-                if !db.is_autocommit() {
-                    // Keep the lock and call implicit_commit directly to avoid race conditions
-                    db.implicit_commit(vm)?;
+                    // If setting isolation_level to None (auto-commit mode), commit any pending transaction
+                    if value.is_none() {
+                        let db = self.db_lock(vm)?;
+                        if !db.is_autocommit() {
+                            // Keep the lock and call implicit_commit directly to avoid race conditions
+                            db.implicit_commit(vm)?;
+                        }
+                    }
+                    let _ = unsafe { self.isolation_level.swap(value) };
+                    Ok(())
                 }
+                PySetterValue::Delete => Err(vm.new_attribute_error(
+                    "'isolation_level' attribute cannot be deleted".to_owned(),
+                )),
             }
-
-            let _ = unsafe { self.isolation_level.swap(val) };
-            Ok(())
         }
 
         #[pygetset]