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Generate genotyping calls from CEL files #2

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dauss75 opened this issue Aug 21, 2015 · 6 comments
Closed

Generate genotyping calls from CEL files #2

dauss75 opened this issue Aug 21, 2015 · 6 comments

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@dauss75
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dauss75 commented Aug 21, 2015

Hello there,

Thanks for the nice tutorial!

I have calvin CEL files that were converted to text files using apt-cel-convert.
Having the text CEL files, I tried to run the command and got an error:
apt-probeset-genotype -c lib/GenomeWideSNP_6.cdf -a birdseed --read-models-birdseed lib/GenomeWideSNP_6.birdseed.models --special-snps lib/GenomeWideSNP_6.specialSNPs --out-dir apt --cel-files listfile

FATAL ERROR:TsvFile.cpp:2672: This file appears to be binary. (filename='../data/test.cel/1.CEL')

It's clear that the CEL files are not binary.
It seems the error is from birdseed, but I was wondering whether you have any thoughts on this.

Thanks,
Segun

@kaichop
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kaichop commented Aug 21, 2015

you can run Linux command "file" to check whether it is binary or text
file. Also delete this file from listfile and see whether other files have
similar issues.

On Fri, Aug 21, 2015 at 8:31 AM, Segun J [email protected] wrote:

Hello there,

Thanks for the nice tutorial!

I have calvin CEL files that were converted to text files using
apt-cel-convert.
Having the text CEL files, I tried to run the command and got an error:
apt-probeset-genotype -c lib/GenomeWideSNP_6.cdf -a birdseed
--read-models-birdseed lib/GenomeWideSNP_6.birdseed.models --special-snps
lib/GenomeWideSNP_6.specialSNPs --out-dir apt --cel-files listfile

FATAL ERROR:TsvFile.cpp:2672: This file appears to be binary.
(filename='../data/test.cel/1.CEL')

It's clear that the CEL files are not binary.

It seems the error is from birdseed, but I was wondering whether you have
any thoughts on this.

Thanks,
Segun


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#2.

@dauss75
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dauss75 commented Aug 21, 2015

thanks for the quick reply.
I am running with only four CEL files and using the "file" command, I get:
1.CEL: data

@kaichop
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kaichop commented Aug 21, 2015

The file may be corrupt, try other CEL files to see if the same thing
occurs for other files. CEL should be a text file.

On Fri, Aug 21, 2015 at 9:04 AM, Segun J [email protected] wrote:

thanks for the quick reply.
I am running with only four CEL files and using the "file" command, I get:
1.CEL: data


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#2 (comment)
.

@dauss75
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dauss75 commented Aug 21, 2015

I figured out the CEL files were generated based on the CytoScanHD_Array platform. Trying to convert them now.

@kaichop kaichop closed this as completed Nov 10, 2015
@Vaidhy-M
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Hello there,

Thanks for the nice tutorial!

I have calvin CEL files that were converted to text files using apt-cel-convert.
Having the text CEL files, I tried to run the command and got an error:
apt-probeset-genotype -c lib/GenomeWideSNP_6.cdf -a birdseed --read-models-birdseed lib/GenomeWideSNP_6.birdseed.models --special-snps lib/GenomeWideSNP_6.specialSNPs --out-dir apt --cel-files listfile

FATAL ERROR:TsvFile.cpp:2672: This file appears to be binary. (filename='../data/test.cel/1.CEL')

It's clear that the CEL files are not binary.
It seems the error is from birdseed, but I was wondering whether you have any thoughts on this.

Thanks,
Segun

I am facing the same issue in Mapping 500K CEL files. Linux File command says the files are in Data format. Could you please explain how to fix this? Should I convert CEL files to a different format? Thanks!

@kaichop
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kaichop commented Feb 15, 2019 via email

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