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We use baker's yeast to study the process underlying meiosis and meiotic recombination. We are particularly interested in the surveillance mechanisms, or checkpoints, that provide the regulatory framework for the step-by-step progression of many meiotic events.</font></p>
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<p>Mansisidor AR, Molinar Jr. T, Srivastava P, Blitzblau H, Klein H, Hochwagen A. (2018) <a href="https://www.biorxiv.org/content/early/2018/01/29/255471"><font color="black">Genomic copy-number loss is rescued by self-limiting production of DNA circles.</font></a> bioRxiv:255471</p>
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<p>Paul MR, Markowitz TE, Hochwagen A, Ercan S. (2017) <a href="https://www.biorxiv.org/content/early/2017/09/28/195487"><font color="black">Acute condensin depletion causes genome decompaction without altering the level of global gene expression in <i>Saccharomyces cerevisiae</i>.</font></a> bioRxiv:195487</p>
</br>
<p>Subramanian VV, Markowitz TM, Vale-Silva LA, San-Segundo P, Hollingsworth N, Hochwagen A. (2017) <a href="https://www.biorxiv.org/content/early/2017/10/11/201889"><font color="black">Persistent DNA-break potential near telomeres increases initiation of meiotic recombination on small chromosomes.</font></a> bioRxiv:201889</p>
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<p>Markowitz TE, Suarez D, Blitzblau HG, Patel NJ, Markhard AL, MacQueen AJ, Hochwagen A. (2017) <a href="publications/2017_PLoSGenet.pdf"><font color="black">Reduced dosage of the chromosome axis factor Red1 selectively disrupts the meiotic recombination checkpoint in <i>Saccharomyces cerevisiae</i>.</font></a> PLoS Genet, 13(7):e1006928</p>
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<h3>Meiotic Checkpoints</h3>
<p align="left" class="text-muted">The meiotic program is orchestrated by a whole network of checkpoint mechanisms. Our research combines molecular biology and systems biology to determine the components and dynamics of this network.</p>
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<h3>Meiotic chromosome structure</h3>
<p align="left" class="text-muted">Meiotic chromosomes undergo profound structural changes including programmed chromosome breakage. We use genomic analyses and chromosome engineering to understand the rules governing chromosome transitions and breakage patterns.</p>
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<h3>2018</h3>
<p>Mansisidor AR, Molinar Jr. T, Srivastava P, Blitzblau H, Klein H, Hochwagen A. (2018) <b>Genomic copy-number loss is rescued by self-limiting production of DNA circles.</b> bioRxiv:255471.
<a href="https://www.biorxiv.org/content/early/2018/01/29/255471">bioRxiv</a></p>
<h3>2017</h3>
<p>Paul MR, Markowitz TE, Hochwagen A, Ercan S. (2017) <b>Acute condensin depletion causes genome decompaction without altering the level of global gene expression in <i>Saccharomyces cerevisiae</i>.</b> bioRxiv:195487.
<a href="https://www.biorxiv.org/content/early/2017/09/28/195487">bioRxiv</a></p>
<p>Subramanian VV, Markowitz TM, Vale-Silva LA, San-Segundo P, Hollingsworth N, Hochwagen A. (2017) <b>Persistent DNA-break potential near telomeres increases initiation of meiotic recombination on small chromosomes.</b> bioRxiv:201889.
<a href="https://www.biorxiv.org/content/early/2017/10/11/201889">bioRxiv</a></p>
<p>Markowitz TE, Suarez D, Blitzblau HG, Patel NJ, Markhard AL, MacQueen AJ, Hochwagen A. (2017) <b>Reduced dosage of the chromosome axis factor Red1 selectively disrupts the meiotic recombination checkpoint in <i>Saccharomyces cerevisiae</i>.</b> PLoS Genet, 13(7):e1006928
<a href="http://www.ncbi.nlm.nih.gov/pubmed/28746375">PubMed</a>
<a href="publications/2017_PLoSGenet.pdf">PDF</a></p>
<p>Argunhan B, Leung WK, Afshar N, Terentyev Y, Subramanian VV, Murayama Y, Hochwagen A, Iwasaki H, Tsubouchi T, Tsubouchi H. (2017) <b>Fundamental cell cycle kinases collaborate to ensure timely destruction of the synaptonemal complex during meiosis.</b> EMBO J, 36(17):2488-2509
<a href="http://www.ncbi.nlm.nih.gov/pubmed/28694245">PubMed</a>
<h3>2016</h3>
<p>Subramanian VV, MacQueen AJ, Vader G, Shinohara M, Sanchez A, Borde V, Shinohara A, Hochwagen A. (2016) <b>Chromosome Synapsis Alleviates Mek1-Dependent Suppression of Meiotic DNA Repair.</b> PLoS Biol, 14(2):e1002369
<a href="http://www.ncbi.nlm.nih.gov/pubmed/26870961">PubMed</a>
<a href="publications/2016_PLoSBiol.pdf">PDF</a></p>
<p>Wang D, Mansisidor A, Prabhakar G, Hochwagen A. (2016) <b>Condensin and Hmo1 Mediate a Starvation-Induced Transcriptional Position Effect within the Ribosomal DNA Array.</b> Cell Rep, 14:1010-1017
<a href="http://www.ncbi.nlm.nih.gov/pubmed/26832415">PubMed</a>
<a href="publications/2016_CellReports.pdf">PDF</a></p>
<p>He H, Zhang S, Wang D, Hochwagen A, Li F. (2016) <b>Condensin Promotes Position Effects within Tandem DNA Repeats via the RITS Complex.</b> Cell Rep, 14:1018-1024
<a href="http://www.ncbi.nlm.nih.gov/pubmed/26832414">PubMed</a>
<a href="publications/2016_CellReportsB.pdf">PDF</a></p>
<h3>2015</h3>
<p>Vincenten N, Kuhl LM, Lam I, Oke A, Kerr AR, Hochwagen A, Fung J, Keeney S, Vader G, Marston AL. (2015) <b>The kinetochore prevents centromere-proximal crossover recombination during meiosis.</b> eLife 2015;4:e10850
<a href="http://www.ncbi.nlm.nih.gov/pubmed/26653857">PubMed</a>
<a href="publications/2015_eLifeB.pdf">PDF</a></p>
<p>Gothwal SK, Patel NJ, Colletti MM, Sasanuma H, Shinohara M, Hochwagen A, Shinohara A. (2015) <b>The Double-Strand Break Landscape of Meiotic Chromosomes Is Shaped by the Paf1 Transcription Elongation Complex in <i>Saccharomyces cerevisiae</i>.</b> Genetics, 202(2):497-512
<a href="http://www.ncbi.nlm.nih.gov/pubmed/26627841">PubMed</a></p>
<p>Sun X, Huang L, Markowitz TE, Blitzblau HG, Chen D, Klein F, Hochwagen A. (2015) <b>Transcription dynamically patterns the meiotic chromosome-axis interface.</b> eLife 2015;4:e07424
<a href="http://www.ncbi.nlm.nih.gov/pubmed/26258962">PubMed</a>
<a href="publications/2015_eLife.pdf">PDF</a></p>
<p>Voelkel-Meiman K, Johnston C, Thappeta Y, Subramanian VV, Hochwagen A, MacQueen AJ. (2015) Separable Crossover-Promoting and Crossover-Constraining Aspects of Zip1 Activity during Budding Yeast Meiosis. PLoS Genet, 11(6):e1005335
<a href="http://www.ncbi.nlm.nih.gov/pubmed/26114667">PubMed</a>
<a href="publications/2015_PLoSGenet.pdf">PDF</a></p>
<h3>2014</h3>
<p>Humphryes N, Hochwagen A. (2014) <b>A non-sister act: Recombination template choice during meiosis.</b> Exp Cell Res, 15;329(1):53-60
<a href="http://www.ncbi.nlm.nih.gov/pubmed/25158281">PubMed</a></p>
<p>Subramanian VV, Hochwagen A. (2014) <b>The Meiotic Checkpoint Network: Step-by-Step through Meiotic Prophase.</b> Cold Spring Harb Perspect Biol 6(10):a016675
<a href="http://www.ncbi.nlm.nih.gov/pubmed/25274702">PubMed</a></p>
<h3>2013</h3>
<p>Copsey A, Tang S, Jordan PW, Blitzblau HG, Newcombe S, et al. (2013) <b>Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions.</b> PLoS Genet 9(12): e1004071
<a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=Smc5%2F6+Coordinates+Formation+and+Resolution+of+Joint+Molecules+with+Chromosome+Morphology+to+Ensure+Meiotic+Divisions">PubMed</a> <a href="publications/2013_plos.pdf">PDF</a></p>
<p>Blitzblau HG, Hochwagen A (2013) <b>ATR/Mec1 prevents lethal meiotic recombination initiation on partially replicated chromosomes in budding yeast.</b> eLife 2013;2:e00844
<a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=ATR%2FMec1+prevents+lethal+meiotic+recombination+initiation+on+partially+replicated+chromosomes+in+budding+yeast">PubMed</a> <a href="publications/2013_elife.pdf">PDF</a></p>
<h3>2012</h3>
<p>Agarwala SD, Blitzblau HG, Hochwagen A, Fink GR (2012) <b>RNA Methylation by the MIS Complex Regulates a Cell Fate Decision in Yeast.</b> PLoS Genet 8(6): e1002732.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/22685417">PubMed</a> <a href="Papers/Agarwala.pdf">PDF</a></p>
<p>Blitzblau HG, Chan CS, Hochwagen A, Bell SP (2012) <b>Separation of DNA Replication from the Assembly of Break- Component Meiotic Chromosomes.</b> PLoS Genet 8(5): e1002643.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/22615576">PubMed</a> <a href="Papers/journal.pgen.1002643.pdf">PDF</a></p>
<h3>2011</h3>
<p> Subramanian, V.V., and Hochwagen, A. (2011). <b>Centromere clustering: where synapsis begins.</b> Curr Biol 21, R920-922.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/22115459">PubMed</a></p>
<p> Vader, G., Blitzblau, H.G., Tame, M.A., Falk, J.E., Curtin, L., and Hochwagen, A. (2011). <b>Protection of repetitive DNA borders from self-induced meiotic instability.</b> Nature 477, 115-119.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/21822291">PubMed</a> <a href="publications/2011_Nature.pdf">PDF</a> <a href="publications/2011_Nature-supp.pdf">Supplemental Data PDF</a></p>
<p> Blitzblau, H.G., and Hochwagen, A. (2011). <b>Genome-wide detection of meiotic DNA double-strand break hotspots using single-stranded DNA.</b> Methods Mol Biol 745, 47-63.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/21660688">PubMed</a> <a href="publications/2011_Methods.pdf">PDF</a></p>
<p> MacQueen, A.J., and Hochwagen, A. (2011). <b>Checkpoint mechanisms: the puppet masters of meiotic prophase.</b> Trends Cell Biol 21, 393-400.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/21531561">PubMed</a></p>
<p> Pan, J., Sasaki, M., Kniewel, R., Murakami, H., Blitzblau, H.G., Tischfield, S.E., Zhu, X., Neale, M.J., Jasin, M., Socci, N.D., Hochwagen, A., Keeney, S. (2011). <b>A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation.</b> Cell 144, 719-731.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/21376234">PubMed</a> <a href="publications/2011_Cell.pdf">PDF</a> <a href="publications/2011_Cell-supp.pdf">Supplemental Data PDF</a></p>
<h3>2010</h3>
<p>Falk, J.E., Chan, A.C., Hoffmann, E., and Hochwagen, A. (2010). <b>A Mec1- and PP4-dependent checkpoint couples centromere pairing to meiotic recombination.</b> Dev Cell 19, 599-611.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/20951350">PubMed</a></p>
<p> Hochwagen, A., and Marais, G.A. (2010). <b>Meiosis: a PRDM9 guide to the hotspots of recombination.</b> Curr Biol 20, R271-274.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/20334833">PubMed</a></p>
<h3>2009</h3>
<p> Hochwagen, A. (2009). <b>Meiosis: making a synaptonemal complex just got easier.</b> Curr Biol 19, R849-851.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/19788878">PubMed</a></p>
<p> Brar, G.A., Hochwagen, A., Ee, L.S., and Amon, A. (2009). <b>The multiple roles of cohesin in meiotic chromosome morphogenesis and pairing.</b> Mol Biol Cell 20, 1030-1047.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/19073884">PubMed</a> <a href="publications/2009_MolBio.pdf">PDF</a></p>
<h3>2008</h3>
<p>Chen, S.Y., Tsubouchi, T., Rockmill, B., Sandler, J.S., Richards, D.R., Vader, G., Hochwagen, A., Roeder, G.S., and Fung, J.C. (2008). <b>Global analysis of the meiotic crossover landscape.</b> Dev Cell 15, 401-415.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/18691940">PubMed</a> <a href="publications/2008_DevCell.pdf">PDF</a> <a href="publications/2008_DevCell-supp.pdf">Supplemental Data PDF</a></p>
<p> Hochwagen, A. (2008). <b>Meiosis.</b> Curr Biol 18, R641-R645.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/18682199">PubMed</a></p>
<h3>2007</h3>
<p> Blitzblau, H.G., Bell, G.W., Rodriguez, J., Bell, S.P., and Hochwagen, A. (2007). <b>Mapping of meiotic single-stranded DNA reveals double-stranded-break hotspots near centromeres and telomeres.</b> Curr Biol 17, 2003-2012.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/18060788">PubMed</a></p>
<h3>2006</h3>
<p> Hochwagen, A., and Amon, A. (2006). <b>Checking your breaks: surveillance mechanisms of meiotic recombination.</b> Curr Biol 16, R217-228.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/16546077">PubMed</a></p>
<h3>2005</h3>
<p>Hochwagen, A., Tham, W.H., Brar, G.A., and Amon, A. (2005). <b>The FK506 binding protein Fpr3 counteracts protein phosphatase 1 to maintain meiotic recombination checkpoint activity.</b> Cell 122, 861-873.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/16179256">PubMed</a></p>
<p>Hochwagen, A., Wrobel, G., Cartron, M., Demougin, P., Niederhauser-Wiederkehr, C., Boselli, M.G., Primig, M., and Amon, A. (2005). <b>Novel response to microtubule perturbation in meiosis.</b> Mol Cell Biol 25, 4767-4781.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/15899877">PubMed</a> <a href="publications/2005_MolBio.pdf">PDF</a></p>
<h3>2002</h3>
<p> Haering, C.H., Lowe, J., Hochwagen, A., and Nasmyth, K. (2002). <b>Molecular architecture of SMC proteins and the yeast cohesin complex.</b> Mol Cell 9, 773-788.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/11983169">PubMed</a></p>
<h3>2000</h3>
<p> Panizza, S., Tanaka, T., Hochwagen, A., Eisenhaber, F., and Nasmyth, K. (2000). <b>Pds5 cooperates with cohesin in maintaining sister chromatid cohesion.</b> Curr Biol 10, 1557-1564.
<a href="http://www.ncbi.nlm.nih.gov/pubmed/11137006">PubMed</a></p>
</div>
</div>
</div>
</section>
</section>
<section class="bg-dark" id="labmembers">
<div class="container">
<div class="row">
<div class="col-lg-8 col-lg-offset-2 text-center">
<h1 class="section-heading">Lab members</h1>
</div>
<div class="col-lg-8 col-lg-offset-2">
<hr class="primary">
<p> </p>
<h3>Principal investigator</h3>
<p> </p>
<table style="width:100%">
<tr>
<td><img class="img-circle-small" src="img/lab_members/Andreas.gif" alt="Andreas" /></td>
<td><p>Andreas Hochwagen, PhD</br>
<strong>Principal Investigator</strong></br>
Assistant Professor of Biology</br>
[email protected]</p></td>
</tr>
<tr><td colspan="2"><p> </p></td></tr>
<tr><td colspan="2"><h3>PostDocs</h3></td></tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/viji_crop.gif" alt="Viji" /></td>
<td><p>Viji Subramanian, PhD</br>
<strong>Postdoc</strong></br>
[email protected]</br></br>
Research interests: Investigating the role of the synaptonemal complex in the regulation of the double-strand break repair outcome.</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Luis_scaled.jpg" alt="Luis" /></td>
<td><p>Luis A. Vale Silva, PhD</br>
<strong>Postdoc</strong></br>
[email protected]</br></br>
Research interests: Investigating the effect of chromosome size on meiotic double-strand break formation.</p></td>
</tr>
<tr><td colspan="2"><h3>Graduate Students</h3></td></tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Tovah.gif" alt="Tovah" /></td>
<td><p>Tovah Markowitz</br>
<strong>PhD student</strong></br>
[email protected]</br></br>
Research interests: Understanding how condensin and cohesin control meiotic chromosome structure and recombination.</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Danni.gif" alt="Danni" /></td>
<td><p>Danni Wang</br>
<strong>PhD student</strong></br>
[email protected]</br></br>
Research interests: Analyzing position effects of the repetitive rDNA array.</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Fluffy.gif" alt="Andres" /></td>
<td><p>Andres Mansisidor</br>
<strong>PhD student</strong></br>
[email protected]</br></br>
Research interests: Investigating the structural and functional dynamics of repetitive DNA and the physiological consequences of such dynamics.</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Matt.gif" alt="Matt" /></td>
<td><p>Matthew Robert Paul</br>
<strong>PhD student</strong></br>
[email protected]</br></br>
Research interests: The role of the 3D organization of chromatin on meiotic double-strand break formation.</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/jonna_scaled.jpg" alt="Jonna" /></td>
<td><p>Jonna Heldrich</br>
<strong>PhD student</strong></br>
[email protected]</br></br>
Research interests: Understanding the role of genome topology in the formation of meiotic double strand breaks.</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Temi.JPG" alt="Temi" /></td>
<td><p><strong>Temi</strong>stocles Molinar Jr.</br>
<strong>PhD student</strong></br>
[email protected]</br></br>
Research interests: Causes and consequences of ribosomal DNA array instability.</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Funda.jpg" alt="Victor" /></td>
<td><p>Funda Mujgan Kar</br>
<strong>PhD student</strong></br>
[email protected]</br></br>
Research interests: Understanding the role of posttranslational modifications in meiosis.</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Charlene.jpg" alt="Charlene" /></td>
<td><p>Charlene Valdez</br>
<strong>Master's student & Technician</strong></br>
[email protected]</br></br>
Research interests: Control of pre-meiotic DNA replication.</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Victor.jpg" alt="Victor" /></td>
<td><p>Victor A. Leon</br>
<strong>Master's student</strong></br>
[email protected]</br></br>
Research interests: Characterization of phosphorylation of the synapsis protein Zip1.</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Ninkka.JPG" alt="Ninkka" /></td>
<td><p>Ninkka Tamot</br>
<strong>Master's student</strong></br>
[email protected]</br></br>
Research interests: Structural and functional analysis of the high-mobility group protein Hmo1.</p></td>
</tr>
<tr><td colspan="2"><h3>Undergraduate students</h3></td></tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Vivian.JPG" alt="Vivian" /></td>
<td><p>Vivian Utti</br>
<strong>Undergraduate student</strong></br>
[email protected]</br></p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Michelle.JPG" alt="Michelle" /></td>
<td><p>Michelle Shi</br>
<strong>Undergraduate student</strong></br>
[email protected]</br></p></td>
</tr>
<tr><td colspan="2"><h3>Former lab members</h3></td></tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Hannah.gif" alt="Hanna" /></td>
<td><p>Hannah Blitzblau, PhD</br>
<strong>Postdoc</strong></br></br>
<strong>Moved on to become</strong></br>
Senior Research Scientist at Novogy</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Gerben_scaled.gif" alt="Gerben" /></td>
<td><p>Gerben Vader, PhD</br>
<strong>Postdoc</strong></br>
<strong>Moved on to become</strong></br>
Junior Group Leader at Max Planck Institute of Molecular Physiology
(<a class="dark" href="http://gerbenvader.wix.com/meiosis">Lab website</a>)</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Neil.gif" alt="Neil" /></td>
<td><p>Neil Humphryes, PhD</br>
<strong>Postdoc</strong></br></br>
<strong>Moved on to become</strong></br>
Senior Bioinformatician at Desktop Genetics, Ltd</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Trevor.JPG" alt="trevor" /></td>
<td><p>Trevor Stoltzfus</br>
<strong>Master's student</strong></br></br>
<strong>Moved on to become</strong></br>
Medical student at Des Moines University</p></td>
<!-- Research interests: Analysis of meiotic chromosome structure protein Hop1.</p></td>-->
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Neem.gif" alt="Neem" /></td>
<td><p>Neem Patel</br>
<strong>Master's student & Technician</strong></br></br>
<strong>Moved on to become</strong></br>
PhD student at UC Berkeley</p></td>
<!-- Research interests: Characterizing features underlying meiotic DSB end resection tracks.</p></td>-->
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Marie.jpg" alt="Marie" /></td>
<td><p>Marie Jezequel</br>
<strong>Visiting Master's student</strong></br></br>
<strong>Moved on to become</strong></br>
Product Manager at Pierre Fabre Laboratories</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/FISH_rDNA.jpg" alt="Jawaher" /></td>
<td><p>Jawaher Al-Zahrani</br>
<strong>Research volunteer</strong></br></br>
<strong>Moved on to become</strong></br>
PhD student at Cornell University</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Daniel_scaled.jpg" alt="Daniel" /></td>
<td><p>Daniel Suarez</br>
<strong>Undergraduate student</strong></br></br>
<strong>Moved on to</strong></br>
Postbaccalaureate program at SUNY Purchase College</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Ashima.jpg" alt="Ashima" /></td>
<td><p>Ashima Goel</br>
<strong>Undergraduate student</strong></br></br>
</br>
</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Priyanka.gif" alt="Priyanka" /></td>
<td><p>Priyanka Srivastava</br>
<strong>Undergraduate student</strong></br></br>
</br>
</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Anna.gif" alt="Anna" /></td>
<td><p>Anna Maria Houshangi</br>
<strong>Master's student & Technician</strong></br></br>
<strong>Moved on to become</strong></br>
NYC Teaching Fellow at Department of Education</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Sunny.gif" alt="Sunny" /></td>
<td><p>Sunny (Xiaoji) Sun</br>
<strong>Master's student</strong></br></br>
<strong>Moved on to become</strong></br>
PhD Student at NYU School of Medicine</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Meaghan_scaled.jpg" alt="Meaghan" /></td>
<td><p>Meaghan Michelle Colletti</br>
<strong>Master's student</strong></br></br>
<strong>Moved on to become</strong></br>
Student at UT Southwestern Medical Center</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Daeun.gif" alt="Daeun" /></td>
<td><p>Daeun Hur</br>
<strong>Undergraduate student</strong></br></br>
<strong>Moved on to become</strong></br>
Student at NYU College of Dentistry</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Raj.gif" alt="Raj" /></td>
<td><p>Raj A. Desai</br>
<strong>Undergraduate student</strong></br></br>
<strong>Moved on to become</strong></br>
Student at Columbia University College of Dental Medicine</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Alisha.gif" alt="Alisha" /></td>
<td><p>Alisha Karim</br>
<strong>Undergraduate student</strong></br></br>
</br>
</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Raghav.jpg" alt="Raghav" /></td>
<td><p>Raghav Mishra</br>
<strong>Undergraduate student</strong></br></br>
</br>
</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Namitha.gif" alt="Namitha" /></td>
<td><p>Namitha L Gubbi</br>
<strong>Undergraduate student</strong></br></br>
</br>
</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Mihoko_scaled.gif" alt="Mihoko" /></td>
<td><p>Mihoko Tame</br>
<strong>Lab Manager & Technician</strong></br></br>
<strong>Moved on to become</strong></br>
PhD student at NKI-AVL</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Jill2.gif" alt="Jill" /></td>
<td><p>Jill Falk</br>
<strong>Lab Manager & Technician</strong></br></br>
<strong>Moved on to become</strong></br>
PhD student at MIT</p></td>
</tr>
<tr>
<td><img class="img-circle-small" src="img/lab_members/Andrew_scaled.gif" alt="Andrew" /></td>
<td><p>Andrew Markhard</br>
<strong>Lab Manager & Technician</strong></br></br>
<strong>Moved on to become</strong></br>
Technician at Mootha Lab in Broad Institute of MIT & Harvard</p></td>
</tr>
</table>
</div>
</div>
</div>
</section>
<section id="protocols">
<div class="container">
<div class="row">
<div class="col-lg-10 col-lg-offset-1 text-center">
<h1 class="section-heading">Protocols</h1>
<hr class="primary">
<p> </p>
</div>
<div class="col-lg-10 col-lg-offset-1">
<p>Genome-wide detection of meiotic DNA double-strand break hotspots using single-stranded DNA.
(<a href="publications/2011_Methods.pdf">PDF</a>)</p>
<p>BYTA Medium Recipe.
(<a href="publications/BYTA_recipe.pdf">PDF</a>)</p>
<p>Dissolving Benomyl in Sporulation Medium.
(<a href="publications/Benomyl.pdf">PDF </a>)</p>
<p> </p>
</div>
</div>
</div>
</section>
<aside class="bg-dark">
<div class="container text-center">
</div>
</aside>
<section id="links">
<div class="container">
<div class="row">
<div class="col-lg-10 col-lg-offset-1 text-center">
<h1 class="section-heading">Links</h1>
<hr class="primary">
<p> </p>
</div>
<div class="col-lg-10 col-lg-offset-1">
<h3 class="section-heading">Current collaborators</h3></br>
<p><strong>Laboratory of Amy MacQueen, Wesleyan University:</strong>
Synaptonemal complex dynamics
<a href="http://macqueenlab.research.wesleyan.edu/"><i class="fa fa-1x fa-link" aria-hidden="true"></i></a></p>
<p><strong>Laboratory of Jennifer Fung, University of California, San Francisco:</strong>
Genome-wide studies of recombination
<a href="http://funglab.ucsf.edu/"><i class="fa fa-1x fa-link" aria-hidden="true"></i></a></p>
<p><strong>Laboratory of Gerry Fink, Whitehead Institute:</strong>
Epigenetic control and cell surface variation in fungi
<a href="http://wi.mit.edu/people/faculty/fink"><i class="fa fa-1x fa-link" aria-hidden="true"></i></a></p>
<p><strong>Laboratory of Eva Hoffmann, University of Sussex, UK:</strong>
Control of meiotic recombination and chromosome segregation
<a href="http://www.sussex.ac.uk/lifesci/hoffmannlab/"><i class="fa fa-1x fa-link" aria-hidden="true"></i></a></p>
<p><strong>Laboratory of Nancy Hollingsworth, State University of New York, Stony Brook:</strong>
DNA repair template choice in meiosis
<a href="http://www.stonybrook.edu/commcms/biochem/research/faculty/hollingsworth/"><i class="fa fa-1x fa-link" aria-hidden="true"></i></a></p>
<p> </p>
</div>
<div class="col-lg-10 col-lg-offset-1">
<h3 class="section-heading">Resources</h3></br>
<p><strong> SK1 (<a href="https://www.ncbi.nlm.nih.gov/pubmed/28416820">Yue <i>et al</i>. 2017</a>) BLAST</strong>
<a href="https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&PROG_DEF=blastn&BLAST_SPEC=Assembly&ASSEMBLY_NAME=GCA_002057885.1"><i class="fa fa-1x fa-link" aria-hidden="true"></i></a></p>
<p><strong>SK1 BLAST</strong>
<a href="http://steinmetzlab.embl.de/blast/"><i class="fa fa-1x fa-link" aria-hidden="true"></i></a></p>
<p><strong>Keeney SK1 sequence</strong>
<a href="http://cbio.mskcc.org/public/SK1_MvO/"><i class="fa fa-1x fa-link" aria-hidden="true"></i></a></p>
<p><strong>Saccharomyces Genome Database (SGD)</strong>
<a href="http://www.yeastgenome.org/"><i class="fa fa-1x fa-link" aria-hidden="true"></i></a></p>
<p><strong>NCBI</strong>
<a href="https://www.ncbi.nlm.nih.gov/"><i class="fa fa-1x fa-link" aria-hidden="true"></i></a></p>
<p> </p>
</div>
</div>
</div>
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<h2 class="section-heading">Contact us</h2>
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<p>Andreas Hochwagen, PhD</br>
Principal Investigator</br>
Assistant Professor of Biology</br>
<i class="fa fa-envelope-o fa-2x sr-contact"></i> [email protected]</br>
<i class="fa fa-phone fa-2x sr-contact"></i> Lab: 1-(212)-992-6556</br>
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<p>Hochwagen Lab</br>
New York University</br>
Department of Biology</br>
1009 Silver Center</br>
100 Washington Square East</br>
New York, NY 10003-6688</br>
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<h3 class="section-heading">Lab shipping address</h3>
<p>Hochwagen Lab</br>
New York University</br>
Department of Biology</br>
150 Brown</br>
100 Washington Square East</br>
New York, NY 10003</br>
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