methods_html/scMandT.html

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<h1><a href="https://www.nature.com/articles/nmeth.3728" target="_blank">scM&T-seq</a></h1>

<p><span style="font-size:1.1em;">The scM&T-seq method was published by Angermueller <i>et al.</i> in Nature Methods 13, 229–232. It uses oligo-dT magnetic beads to drag mRNA out to achieve phycical separation of mRNA and genomic DNA. For the mRNA part, <a href="https://www.nature.com/nmeth/journal/v10/n11/full/nmeth.2639.html" target="_blank">SMART-seq2</a> method was used for scRNA-seq, <a href="https://teichlab.github.io/scg_lib_structs/methods_html/SMART-seq_family.html" target="_blank">click here</a> to see the step-by-step protocol; for the genomic DNA part, <a href="https://www.nature.com/articles/nmeth.3035" target="_blank">scBS-seq</a> method was used to profile methylation, and <a href="https://teichlab.github.io/scg_lib_structs/methods_html/scBS-seq.html" target="_blank">click here</a> to see the step-by-step protocol.</span></p>

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