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Not running #250

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rohenriques opened this issue Nov 13, 2019 · 1 comment
Open

Not running #250

rohenriques opened this issue Nov 13, 2019 · 1 comment

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@rohenriques
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Hello, I'm trying to use MegaHit for the first time in our cluster and I can't seem to make it run.
It keeps failing at the same point:

megahit -t 4 --k-list 23,51,79 -1 PNN_R1_norm.fq,PCN_R1_norm.fq,POR_R1_norm.fq,PWC_R1_norm.fq,PSW_R1_norm.fq -2 PNN_R2_norm.fq,PCN_R2_norm.fq,POR_R2_norm.fq,PWC_R2_norm.fq,PSW_R2_norm.fq -o paradoxus_mega_out
503.0Gb memory in total.
Using: 453.422Gb.
MEGAHIT v1.0.4-beta-4-g58b0995
--- [Wed Nov 13 14:48:22 2019] Start assembly. Number of CPU threads 4 ---
--- [Wed Nov 13 14:48:22 2019] Available memory: 540952932352, used: 486857639116
--- [Wed Nov 13 14:48:22 2019] k list: 23,51,79 ---

And it doesn't give me an error message that I can use.
I've tried changing the --k-list (or not including it at all), the memory available, and even the input files, and nothing works.
The weird thing is that it did work in the same cluster for one of my colleagues.

Any suggestions?

Script:
cd $PBS_O_WORKDIR

module load app/megahit/1.0.4-beta-58b0995

megahit -t 4 --k-list 23,51,79 -1 PNN_R1_norm.fq,PCN_R1_norm.fq,POR_R1_norm.fq,PWC_R1_norm.fq,PSW_R1_norm.fq -2 PNN_R2_norm.fq,PCN_R2_norm.fq,POR_R2_norm.fq,PWC_R2_norm.fq,PSW_R2_norm.fq -o paradoxus_mega_out2

Thank you!!

@voutcn
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voutcn commented Feb 23, 2020

No idea why this happened. You could confirm whether it is running with Linux tools like htop or top.
Nonetheless, I recommend using the latest version. V1.0.4 is too old.

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