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Hello, I'm trying to use MegaHit for the first time in our cluster and I can't seem to make it run.
It keeps failing at the same point:
megahit -t 4 --k-list 23,51,79 -1 PNN_R1_norm.fq,PCN_R1_norm.fq,POR_R1_norm.fq,PWC_R1_norm.fq,PSW_R1_norm.fq -2 PNN_R2_norm.fq,PCN_R2_norm.fq,POR_R2_norm.fq,PWC_R2_norm.fq,PSW_R2_norm.fq -o paradoxus_mega_out
503.0Gb memory in total.
Using: 453.422Gb.
MEGAHIT v1.0.4-beta-4-g58b0995
--- [Wed Nov 13 14:48:22 2019] Start assembly. Number of CPU threads 4 ---
--- [Wed Nov 13 14:48:22 2019] Available memory: 540952932352, used: 486857639116
--- [Wed Nov 13 14:48:22 2019] k list: 23,51,79 ---
And it doesn't give me an error message that I can use.
I've tried changing the --k-list (or not including it at all), the memory available, and even the input files, and nothing works.
The weird thing is that it did work in the same cluster for one of my colleagues.
No idea why this happened. You could confirm whether it is running with Linux tools like htop or top.
Nonetheless, I recommend using the latest version. V1.0.4 is too old.
Hello, I'm trying to use MegaHit for the first time in our cluster and I can't seem to make it run.
It keeps failing at the same point:
megahit -t 4 --k-list 23,51,79 -1 PNN_R1_norm.fq,PCN_R1_norm.fq,POR_R1_norm.fq,PWC_R1_norm.fq,PSW_R1_norm.fq -2 PNN_R2_norm.fq,PCN_R2_norm.fq,POR_R2_norm.fq,PWC_R2_norm.fq,PSW_R2_norm.fq -o paradoxus_mega_out
503.0Gb memory in total.
Using: 453.422Gb.
MEGAHIT v1.0.4-beta-4-g58b0995
--- [Wed Nov 13 14:48:22 2019] Start assembly. Number of CPU threads 4 ---
--- [Wed Nov 13 14:48:22 2019] Available memory: 540952932352, used: 486857639116
--- [Wed Nov 13 14:48:22 2019] k list: 23,51,79 ---
And it doesn't give me an error message that I can use.
I've tried changing the --k-list (or not including it at all), the memory available, and even the input files, and nothing works.
The weird thing is that it did work in the same cluster for one of my colleagues.
Any suggestions?
Script:
cd $PBS_O_WORKDIR
module load app/megahit/1.0.4-beta-58b0995
megahit -t 4 --k-list 23,51,79 -1 PNN_R1_norm.fq,PCN_R1_norm.fq,POR_R1_norm.fq,PWC_R1_norm.fq,PSW_R1_norm.fq -2 PNN_R2_norm.fq,PCN_R2_norm.fq,POR_R2_norm.fq,PWC_R2_norm.fq,PSW_R2_norm.fq -o paradoxus_mega_out2
Thank you!!
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