The aim of this project was to develop and build a ‘modular bioreactor for muscle tissue engineering’ prototype in collaboration with New Harvest. The provided solution should be well documented open-source, easy and low-cost to rebuild, without compromising on functionality. The finished prototype should contain:
- a stand-alone incubation system with:
- temperature and CO2 concentration control,
- housing, power supply and
- open ports for sensory and regulatory I/O of additional modules,
- tray modules holding:
- a controllable perfusion system,
- gas exchange and air bubble removal, as well as
- customizable tissue platforms for biological samples
- System functionality
- Specifications
- Incubator operation instructions
- Tray operation instructions
- Licence
The bioreactor operates on two levels. The incubator is an enclosed unit which allows regulation of internal environmental conditions, currently temperature and CO2. The regulatory circuit runs on an ESP32 microcontroller, which allows operation via display and buttons, as well as a web interface and android/iphone app. Parameters can be changed, monitored and logged. Each tray has an additional microcontroller, for regulation of flow rate and open ports for additional sensorics and regulation.
All our projects are as usefully open-source as possible.
Hardware including documentation is licensed under CERN OHL v.1.2. license
Firmware and software originating from the project is licensed under GNU GENERAL PUBLIC LICENSE v3.
Open data generated by our projects is licensed under CC0.
All our websites and additional documentation are licensed under [Creative Commons Attribution-ShareAlike 4 .0 Unported License] (https://creativecommons.org/licenses/by-sa/4.0/legalcode).
What this means is that you can use hardware, firmware, software and documentation without paying a royalty and knowing that you'll be able to use your version forever. You are also free to make changes but if you share these changes then you have to do so on the same conditions that you enjoy.
Symbiolab, Koruza, GoodEnoughCNC and IRNAS are all names and marks of Institut IRNAS Rače. You may use these names and terms only to attribute the appropriate entity as required by the Open Licences referred to above. You may not use them in any other way and in particular you may not use them to imply endorsement or authorization of any hardware that you design, make or sell.