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Berlin K, Koren S, Chin CS, Drake PJ, Landolin JM, Phillippy AM Assembling Large Genomes with Single-Molecule Sequencing and Locality Sensitive Hashing. Nature Biotechnology. (2015). | ||
Stay tuned for a Canu-specific citation |
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Software Background | ||
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Canu is derived from the Celera Assembler. The Celera assembler [Myers 2000] | ||
was designed to reconstruct mammalian | ||
chromosomal DNA sequences from the short fragments of a whole genome shotgun | ||
sequencing project. The Celera Assembler was used to produce reconstructions of | ||
several large genomes, namely those of Homo sapiens [Venter 2001], Mus musculus | ||
[Mural 2002], Rattus norvegicus [unpublished data], Canis familiaris [Kirkness | ||
2003], Drosophila melanogaster [Adams 2000], and Anopheles gambiae [Holt 2001]. | ||
The Celera Assembler was shown to be very accurate when its reconstruction of | ||
the human genome was compared to independent reconstructions completed later | ||
[Istrail 2004]. It was used to reconstructing one of the first large-scale | ||
metagenomic projects [Venter 2004, Rusch 2007] and a diploid human reference [Levy 2007, Denisov 2008]. | ||
It was adapted to 454 Pyrosequencing [Miller 2008] and PacBio sequencing [Koren 2011], demonstrating | ||
finished bacterial genomes [Koren 2013] and efficient algorithms for eukaryotic assembly [Berlin 2015]. | ||
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In 2015 Canu was forked from Celera Assembler and specialized for single-molecule | ||
high-noise sequences. The Celera Assembler codebase is no longer maintained. | ||
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Canu is a pipeline consisting of several executable programs and perl driver scripts. | ||
The source code includes programs in the C++ language with Unix make scripts. The original | ||
Celera Assembler was designed to run under Compaq(R) Tru64(R) Unix with access to 32GB | ||
RAM. It has also run under IBM(R) AIX(R) and Red Hat Linux. | ||
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The Celera Assembler was released under the GNU General Public License, version 2 as as supplement | ||
to the publication [Istrail 2004]. For the most recent license information please see | ||
README.licences | ||
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References | ||
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Adams et al. (2000) The Genome Sequence of Drosophila melanogaster. Science 287 2185-2195. | ||
Myers et al. (2000) A Whole-Genome Assembly of Drosophila. Science 287 2196-2204. | ||
Venter et al. (2001) The Sequence of the Human Genome. Science 291 1304-1351. | ||
Mural et al. (2002) A Comparison of Whole-Genome Shotgun-Derived Mouse Chromosome 16 and the Human Genome. Science 296 1661-1671. | ||
Holt et al. (2002) The Genome Sequence of the Malaria Mosquito Anophelesd gambiae. Science 298 129-149. | ||
Istrail et al. (2004) Whole Genome Shotgun Assembly and Comparison of Human Genome Assemblies. PNAS 101 1916-1921. | ||
Kirkness et al. (2003) The Dog Genome: Survey Sequencing and Comparative Analysis. Science 301 1898-1903. | ||
Venter et al. (2004) Environmental genome shotgun sequencing of the Sargasso Sea. Science 304 66-74. | ||
Levy et al. (2007) The Diploid Genome Sequence of an Individual Human. PLoS Biology 0050254 | ||
Rusch et al. (2007) The Sorcerer II Global Ocean Sampling Expedition: Northwest Atlantic through Eastern Tropical Pacific. PLoS Biology 1821060. | ||
Denisov et al. (2008) Consensus Generation and Variant Detection by Celera Assembler. Bioinformatics 24(8):1035-40 | ||
Miller et al. (2008) Aggressive Assembly of Pyrosequencing Reads with Mates. Bioinformatics 24(24):2818-2824 | ||
Koren et al. (2012) Hybrid error correction and de novo assembly of single-molecule sequencing reads, Nature Biotechnology, July 2012. | ||
Koren et al. (2013) Reducing assembly complexity of microbial genomes with single-molecule sequencing, Genome Biology 14:R101. | ||
Berlin et. al. (2015) Assembling Large Genomes with Single-Molecule Sequencing and Locality Sensitive Hashing. Nature Biotechnology. (2015). |