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Tool for the Quality Control of Long-Read Defined Transcriptomes

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SQANTI3

SQANTI3 is the newest version of the SQANTI tool that merges features from SQANTI and SQANTI2, together with new additions. SQANTI3 will continue as an integrated development aiming to provide the best characterization for your new long read-defined transcriptome.

SQANTI3 is the first module of the Functional IsoTranscriptomics (FIT) framework, which also includes IsoAnnot and tappAS.

Installation

The latest SQANTI3 release (04/10/2023) is version 5.2. See our wiki for installation instructions.

For informacion about previous releases and features introduced in them, see the version history.

WARNING: v5.0 represented a major release of the SQANTI3 software. Versions of SQANTI3 >= 5.0 will not have backward compatibility with previous releases and their output (v4.3 and earlier). Users that wish to apply any of the new functionalities in v5.0 to output files from older versions will herefore need to re-run SQANTI3 QC. See below for a full list of changes implemented in SQANTI3 v5.0.

Documentation

For detailed documentation, please visit the SQANTI3 wiki.

Wiki contents:

Please, note that we are currently updating and expanding the wiki to provide as much information as possible and enhance the SQANTI3 user experience. Pages under construction -or where information is still missing- will be indicated where appropriate. Thank you for your patience!

How to cite SQANTI3

SQANTI3 paper is currently in preparation. In the meantime, when using SQANTI3 in your research, please cite the original SQANTI paper as well as this repository:

  • Tardaguila M, de la Fuente L, Marti C, et al. SQANTI: extensive characterization of long-read transcript sequences for quality control in full-length transcriptome identification and quantification. Genome Res, 2018. 28(3):396-411. doi:10.1101/gr.222976.117

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