Unravelling the cytoskeletal architecture of cancer cells: a novel computational approach to predict cell invasive potential - CODE
This code is divided in 7 chapters:
- Dataset: Inspect the dataset with RGB and 2D deconvoluted cytoskeleton/nuclei images.
- Nuclei Preprocessing: Adjust the parameters for a given image to segment and preprocess nuclei and visualize the results.
- Cytoskeleton Preprocessing: Adjust the parameters for a given image to indentify individual filaments and visualize the results.
- Cell Segmentation: Use ROIpoly to draw a polygonal mask around the cell of interest.
- Processing: Extract line segments and prepare cells for feature extraction.
- Feature Extraction: Extract the desired features (DCFs, LSFs or CNFs).
- Results Analysis: Compare feature values between single or a population of cells.
- Use the
environment.yaml
file to setup a dedicated environment with the required packages to run the code (TO DO).