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Official implementation of the paper: Vieira, D. F. et al. "Unraveling the cytoskeletal architecture of cancer cells: a novel computational approach to predict cell fate", Scientific Reports - Nature (2024)

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diogojfv/aTubulinOrganization

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Unravelling the cytoskeletal architecture of cancer cells: a novel computational approach to predict cell invasive potential - CODE

This code is divided in 7 chapters:

  1. Dataset: Inspect the dataset with RGB and 2D deconvoluted cytoskeleton/nuclei images.
  2. Nuclei Preprocessing: Adjust the parameters for a given image to segment and preprocess nuclei and visualize the results.
  3. Cytoskeleton Preprocessing: Adjust the parameters for a given image to indentify individual filaments and visualize the results.
  4. Cell Segmentation: Use ROIpoly to draw a polygonal mask around the cell of interest.
  5. Processing: Extract line segments and prepare cells for feature extraction.
  6. Feature Extraction: Extract the desired features (DCFs, LSFs or CNFs).
  7. Results Analysis: Compare feature values between single or a population of cells.

Additional notes:

  • Use the environment.yaml file to setup a dedicated environment with the required packages to run the code (TO DO).

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Official implementation of the paper: Vieira, D. F. et al. "Unraveling the cytoskeletal architecture of cancer cells: a novel computational approach to predict cell fate", Scientific Reports - Nature (2024)

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