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Software package for compartmentalized diffusion in STED-FCS microscopy
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jkfindeisen/compartmentalized_diffusion_STED-FCS_2015
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Example software package for "Cortical actin networks induce spatio-temporal confinement of phospholipids in the plasma membrane – a minimally invasive investigation by STED-FCS." by Andrade, D., Clausen, M., Keller, J. et al. Sci Rep 5, 11454 (2015). https://doi.org/10.1038/srep11454 Note: Contains a fast FCS curve calculation, which worked with Matlab-2014b, but does not work anymore with Matlab-2021b. It might be simple to fix, but we cannot fix it right now. 1. Examples of free, trapped and hopping diffusion Call free_example.m, hop_example.m and trap_example.m. Will compute correlation curves (on an old Matlab version). 2. Free, trappend and hopping diffusion simulations See code/free_simulation_trajectory.m, hop_simulation_trajectory.m, trap_simulation_trajectory.m 3. FCS curve computation See code/free_simulation_run, hop_simulation_run.m, trap_simulation_run.m and in particular fcs_corr_xxx.m. Note: Requires compiled C code. Will not run on newer Matlabs (old Matlab 2014 will probably work), newer will not. 4. Fitting of FCS curves to experimental data See hop_fit_experimental_data.m and code/free_simulation_run, hop_simulation_run.m, trap_simulation_run.m and there in particular subfunction anomFCSCurve, the curve License: MIT license (see LICENSE.txt) except for ui_progressbar.m (2-clause BSD) Code is provided as is, no guarantees for anything.
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