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Fixing user guide
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leonardojo committed Sep 19, 2023
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8 changes: 5 additions & 3 deletions ggPlantmap.userguide.Rmd
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Expand Up @@ -19,7 +19,7 @@ ggPlantmap is an open-source R package with the goal of facilitating the generat
Included in the package there is a set of pre-loaded maps created from previously published plant images that can be directly inserted into a ggplot coding workflow. ggPlantmap enables users to plot heatmap signatures of gene expression or any spatial quantitative data onto plant images providing a customizable and extensible platform for visualizing, and analyzing spatial quantitative patterns within specific plant regions This package uses the flexibility of the well-known ggplot2 R package to allow users to tailor maps to their specific research questions.


## 1. Installing ggPlant map
## 1. Installing ggPlantmap

```{r, eval = FALSE}
## ggPlantmap installation requires devtools.
Expand Down Expand Up @@ -176,7 +176,9 @@ ggPlantmap.plot(ggPm.At.leaf.topview,show.legend = F) +
With ggPlantmap you can overlay quantitative data into your ggPlantmap to visualize it as sort of a heatmap.
To do so, you will need another table with contains quantitative data attributed to your ROIs.
<img src="man/figures/guide/Slide7.JPG" align="center" width="1200"/><br />
<b>IMPORTANT!!!!

<b>
### IMPORTANT!!!!

Note that the name of the ROI levels on your map should exactly match the ones on your quantitative data!!!</b>

Expand Down Expand Up @@ -219,7 +221,7 @@ ggPlantmap.heatmap(quant.data,AT5G47670.expression) +

### 4 Create your own ggPlantmap
Hopefully by now you should be a little familiar with the package.
But we encourage you to create your own ggPlantmap.
But I encourage you to create your own ggPlantmap.
To do so, we will require a specific open-source imaging software called Icy (https://icy.bioimageanalysis.org/).
Source image: https://www.mdpi.com/ijms/ijms-16-13989/article_deploy/html/images/ijms-16-13989-g007.png

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11 changes: 6 additions & 5 deletions ggPlantmap.userguide.md
Original file line number Diff line number Diff line change
Expand Up @@ -19,7 +19,7 @@ plant regions This package uses the flexibility of the well-known
ggplot2 R package to allow users to tailor maps to their specific
research questions.

## 1. Installing ggPlant map
## 1. Installing ggPlantmap

``` r
## ggPlantmap installation requires devtools.
Expand Down Expand Up @@ -316,7 +316,8 @@ With ggPlantmap you can overlay quantitative data into your ggPlantmap
to visualize it as sort of a heatmap. To do so, you will need another
table with contains quantitative data attributed to your ROIs.
<img src="man/figures/guide/Slide7.JPG" align="center" width="1200"/><br />
<b>IMPORTANT!!!!

<b> \#\#\# IMPORTANT!!!!

Note that the name of the ROI levels on your map should exactly match
the ones on your quantitative data!!!</b>
Expand Down Expand Up @@ -433,9 +434,9 @@ ggPlantmap.heatmap(quant.data,AT5G47670.expression) +

### 4 Create your own ggPlantmap

Hopefully by now you should be a little familiar with the package. But
we encourage you to create your own ggPlantmap. To do so, we will
require a specific open-source imaging software called Icy
Hopefully by now you should be a little familiar with the package. But I
encourage you to create your own ggPlantmap. To do so, we will require a
specific open-source imaging software called Icy
(<https://icy.bioimageanalysis.org/>). Source image:
<https://www.mdpi.com/ijms/ijms-16-13989/article_deploy/html/images/ijms-16-13989-g007.png>

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