Keun Hong Son1,2,3, and Je-Yoel Cho1,2,3
1 Department of Biochemistry, College of Veterinary Medicine, Seoul National University, Seoul, Korea
2 Comparative Medicine and Disease Research Center (CDRC), Science Research Center (SRC), Seoul National University, Seoul, Korea
3 BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea
bioRxiv (Posted July 22, 2024)
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gencube enables researchers to search for, download, and unify genome assemblies and diverse types of annotations, and retrieve integrated metadata for high-throughput sequencing-based multi-omics resources suitable for specific requirements.
- GenBank: NCBI GenBank Nucleotide Sequence Database
- RefSeq: NCBI Reference Sequence Database
- GenArk: UCSC Genome Archive
- Ensembl Rapid Release: Ensembl genome browser that provides frequent updates for newly sequenced species
- Zoonomia TOGA: Tool to infer Orthologs from Genome Alignments
- INSDC: International Nucleotide Sequence Database Collaboration
- SRA: NCBI Sequence Read Archive
- ENA: EMBL-EBI European Nucleotide Archive
- DDBJ: DNA Data Bank of Japan
- GenBank & RefSeq README.txt -
genome,geneset,sequence - UCSC GenArk paper -
genome,geneset,annotation - Ensembl Rapid Release Help & Docs & Ensembl 2023 paper -
genome,geneset,sequence,crossgenome - Zoonomia TOGA README.txt & Paper -
geneset,crossgenome - Search in SRA Entrez, Entrez Help & SRA Advanced Search Builder -
seqmeta
The latest release can be installed with
$ pip install gencubeAlternative
# Still setting up!!
$ conda install -c bioconda gencubeWhen you first run gencube, you'll be prompted for your email and NCBI API key, which are saved in the .gencube_entrez_info file in your home directory for future use.
All gencube key subcommands use NCBI's Entrez Utilities (E-Utilities), requiring an email. Without an NCBI API key, you can make 3 requests per second; with an NCBI API key, this limit increases to 10 requests per second. If you submit your NCBI API key, you can perform tasks at more than three times the speed when using the seqmeta subcommand, especially when fetching metadata. If possible, it is recommended to submit your API key.
$ gencube
Email address:
NCBI API key (type 'no' to skip):
To update the submitted information, run the following command.
$ gencube info
gencube consists of six main subcommands excluding info
$ gencube
usage: gencube [-h] {genome,geneset,annotation,sequence,crossgenome,seqmeta,info} ...
gencube v1.0.0
positional arguments:
{genome,geneset,annotation,sequence,crossgenome,seqmeta,info}
genome Search, download, and modify chromosome labels for genome assemblies
geneset Search, download, and modify chromosome labels for genesets (gene annotations)
annotation Search, download, and modify chromosome labels for various genome annotations, such as gaps and repeats
sequence Search and download sequence data of genesets
crossgenome Search and download comparative genomics data, such as homology, and codon or protein alignments
seqmeta Search, retrive, and integrate metadata of experimental sequencing data
info Resubmit email and NCBI API key for use with NCBI's Entrez Utilities (E-Utilities)
options:
-h, --help show this help message and exit
0. The positional argument and options shared among the genome, geneset, sequence, annotation, and crossgenome subcommand
When using the above five subcommands, it's important to find genome assemblies required for personal research. Below are the positional argument and options shared by the these subcommands to browse and search for specific genome assemblies.
positional arguments:
keywords Taxonomic names to search for genomes
You can provide various forms such as species names or accession numbers
Examples: homo_sapiens, human, GCF_000001405.40, GCA_000001405.29, GRCh38, hg38
Multiple names can be combined and will be merged in the search results
To specify multiple names, separate them with spaces
options:
-h, --help show this help message and exit
-v level, --level level
Specify the genome assembly level (default: complete,chromosome)
complete : Fully assembled genomes
chromosome : Assembled at the chromosome level
scaffold : Assembled into scaffolds, but not to the chromosome level
contig : Contiguous sequences without gaps
-r, --refseq Show genomes that have RefSeq accession (GCF_* format)
-u, --ucsc Show genomes that have UCSC name
-l, --latest Show genomes corresponding to the latest version
# Search using scientific or common name
# It is recommended to use the scientific name for a more precise search.
$ gencube genome homo_sapiens
$ gencube genome canis_lupus_familiaris
$ gencube genome human # less accurate
$ gencube genome dog # less accurate
# Search using assembly name
$ gencube genome GRCh38
$ gencube genome grch38 # Case sensitivity is not an issue
$ gencube genome GRCm39 GRCm38 # Multiple keywords can be searched simultaneously
# Search using UCSC name
$ gencube genome hg38
$ gencube genome hg38 hg19
$ gencube genome mm39 mm10
$ gencube genome canfam4 canfam5 canfam6
# Search using GenBank (GCA_*) or RefSeq (GCF_*) accession
$ gencube genome GCA_021950905.1
$ gencube genome GCF_000001405.40
$ gencube genome GCF_000001405.40 GCA_021950905.1
# Show searched genomes corresponding to all genome assembly levels
$ gencube genome homo_sapiens --level complete,chromosome # default
$ gencube genome homo_sapiens --level complete,chromosome,scaffold,contig
$ gencube genome homo_sapiens --level scaffold,contig
# Only show genomes that have RefSeq accession and UCSC name, and correspond to the latest version
$ gencube genome homo_sapiens --refseq --ucsc --latest🔥 $ gencube genome GCF_000001405.40 GCA_021950905.1
# Search assemblies in NCBI database
Keyword: ['GCF_000001405.40', 'GCA_021950905.1']
Total 3 genomes are searched.
# Convert JSON to dataframe format.
Filter options
Level: ['Complete', 'Chromosome']
RefSeq: False
UCSC: False
Latest: False
# Check accessibility to GenArk, Ensembl Rapid Release
UCSC GenArk : 4167 genomes across 2813 species
Ensembl Rapid: 2272 genomes across 1522 species
+----+------------------------+---------+------------+------------------+--------+----------+-----------+
| | Assembly name | Taxid | Release | NCBI | UCSC | GenArk | Ensembl |
+====+========================+=========+============+==================+========+==========+===========+
| 0 | HG002.mat.cur.20211005 | 9606 | 2022/02/04 | GCA_021951015.1 | | v | v |
+----+------------------------+---------+------------+------------------+--------+----------+-----------+
| 1 | HG002.pat.cur.20211005 | 9606 | 2022/02/04 | GCA_021950905.1 | | v | v |
+----+------------------------+---------+------------+------------------+--------+----------+-----------+
| 2 | GRCh38.p14 | 9606 | 2022/02/03 | GCF_000001405.40 | hg38 | v | |
+----+------------------------+---------+------------+------------------+--------+----------+-----------+
Search, download, and modify chromosome labels for genome assemblies
You can download genome data in FASTA format from four different databases (GenBank, RefSeq, GenArk, Ensembl Rapid Release). Each database uses a different soft-masking method, and you can selectively download the data as needed. You can also download unmasked and hard-masked genomes from the Ensembl Rapid Release database.
options:
-m, --metadata Save metadata for the searched genomes
-d, --download Download "fasta" formatted genome file
-db types, --database types
Database where genome file is downloaded (default: refseq)
Default is from the RefSeq database
If not available, download from the GenBank database
genbank : by NCBI GenBank
refseq : by NCBI RefSeq
genark : by UCSC GenArk
ensembl : by Ensembl Rapid Release
-c type, --chr_style type
Chromosome label style used in the download file (default: ensembl)
ensembl : 1, 2, X, MT & unknowns (GenBank IDs)
gencode : chr1, chr2, chrX, chrM & unknowns (GenBank IDs)
ucsc : chr1, chr2, chrX, chrM & unknowns (UCSC-specific IDs)
!! Limited use if UCSC IDs are not issued
raw : Uses raw file labels without modification
- NCBI GenBank: CM_* or other-form IDs
- NCBI RefSeq : NC_*, NW_* or other-form IDs
- GenArk : GenBank or RefSeq IDs
- Ensembl : Ensembl IDs
-mk type, --masking type
Masking type for output data (default: soft)
soft : soft-masked
hard : hard-masked
none : unmasked
-cl 1-9, --compresslevel 1-9
Compression level for output data (default: 6)
Lower numbers are faster but have lower compression
--recursive Download files regardless of their presence only if integrity check is not possible
# Download the full information metadata of searched genomes
$ gencube genome homo_sapiens --metadata
$ gencube genome canis_lupus_familiaris --metadata
# Download genome file under the default conditions (RefSeq or GenBank)
$ gencube genome GCF_011100685.1 --download
$ gencube genome GCF_011100685.1 -d
# Download genome file from a specific database
$ gencube genome GCF_011100685.1 --download --database ensemble
# Download multiple genomes from various databases
$ gencube genome GCF_011100685.1 --download --database refseq,genark,ensemble
# Change the chromosome labels
$ gencube genome GCF_011100685.1 --download --chr_style ensembl # default
$ gencube genome GCF_011100685.1 --download --chr_style gencode
$ gencube genome GCF_011100685.1 --download --chr_style ucsc
# Change the masking type
$ gencube genome GCF_011100685.1 --download --masking soft # default
$ gencube genome GCF_011100685.1 --download --masking hard
$ gencube genome GCF_011100685.1 --download --masking none
# Set the compression level of the file to 2.
$ gencube genome GCF_011100685.1 --download --compresslevel 6 # default
$ gencube genome GCF_011100685.1 --download --compresslevel 1🔥 $ gencube genome GCF_011100685.1 --download --chr_style gencode
# Search assemblies in NCBI database
Keyword: ['GCF_011100685.1']
Total 1 genomes is searched
# Filter genomes based on the following criteria
Level: ['Complete', 'Chromosome']
RefSeq: False
UCSC: False
Latest: False
# Check accessibility to GenArk, Ensembl Rapid Release
UCSC GenArk : 5122 genomes across 3389 species
Ensembl Rapid: 2582 genomes across 1788 species
+----+-----------------+---------+------------+-----------------+---------+----------+-----------+
| | Assembly name | Taxid | Release | NCBI | UCSC | GenArk | Ensembl |
+====+=================+=========+============+=================+=========+==========+===========+
| 0 | UU_Cfam_GSD_1.0 | 9615 | 2020/03/10 | GCF_011100685.1 | canFam4 | v | v |
+----+-----------------+---------+------------+-----------------+---------+----------+-----------+
[GCA_011100685.1 / GCF_011100685.1 / UU_Cfam_GSD_1.0]
- refseq
Canis_lupus_familiaris-UU_Cfam_GSD_1.0_assembly_report.txt: 263kB [00:00, 673kB/s]
Canis_lupus_familiaris-UU_Cfam_GSD_1.0-refseq.sm.fa.gz: 100%|████████████████████████████| 742M/742M [02:39<00:00, 4.88MB/s]
# Change chromosome names and masking method: gencode-style & soft-masked
[GCA_011100685.1 / GCF_011100685.1 / UU_Cfam_GSD_1.0]
Downloaded genome: ['refseq.sm']
- refseq.sm
Modify chromosome names
Write compressed fasta file (compresslevel: 6)
Processing time: 455 seconds
!! If the file appears to have any problems, please delete it and retry the process
Search, download, and modify chromosome labels for genesets (gene annotations)
options:
-d types, --download types
Type of gene set
refseq_gtf : RefSeq gene set (GTF format)
refseq_gff : RefSeq gene set (GFF)
gnomon : RefSeq Gnomon gene prediction (GFF)
cross : RefSeq Cross-species alignments (GFF)
same : RefSeq Same-species alignments (GFF)
agustus : GenArk Augustus gene prediction (GFF)
xenoref : GenArk XenoRefGene (GFF)
genark_ref : GenArk RefSeq gene models (GFF)
ensembl_gtf : Ensembl Rapid Release gene set (GTF)
ensembl_gff : Ensembl Rapid Release gene set (GFF)
toga_gtf : Zoonomia TOGA gene set (GTF)
toga_bed : Zoonomia TOGA gene set (BED)
toga_pseudo : Zoonomia TOGA processed pseudogenes (BED)
-c type, --chr_style type
Chromosome label style used in the download file (default: ensembl)
ensembl : 1, 2, X, MT & unknowns (GenBank IDs)
gencode : chr1, chr2, chrX, chrM & unknowns (GenBank IDs)
ucsc : chr1, chr2, chrX, chrM & unknowns (UCSC-specific IDs)
!! Limited use if UCSC IDs are not issued
raw : Uses raw file labels without modification
- NCBI GenBank: CM_* or other-form IDs
- NCBI RefSeq : NC_*, NW_* or other-form IDs
- GenArk : GenBank or RefSeq IDs
- Ensembl : Ensembl IDs
--recursive Download files regardless of their presence only if integrity check is not possible
# search usable and accessible data
gencube geneset canis_lupus_familiaris
gencube geneset GCF_011100685.1
# Download specific geneset file from a database
$ gencube geneset GCF_011100685.1 --download refseq_gtf
$ gencube geneset GCF_011100685.1 --download agustus
$ gencube geneset GCF_011100685.1 --download toga_gtf
# Download multiple genesets from various databases
$ gencube geneset GCF_011100685.1 --download refseq_gtf,agustus,toga_gtf🔥 $ gencube geneset canis_lupus_familiaris
# Search assemblies in NCBI database
Keyword: ['canis_lupus_familiaris']
Total 34 genomes are searched
# Filter genomes based on the following criteria
Level: ['Complete', 'Chromosome']
RefSeq: False
UCSC: False
Latest: False
# Check accessibility to GenArk, Ensembl Rapid Release and Zoonomia server
UCSC GenArk : 5122 genomes across 3389 species
Ensembl Rapid: 2582 genomes across 1788 species
Zoonomia TOGA: 951 genomes across 973 species
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
| | Assembly name | Taxid | Release | NCBI | UCSC | GenArk | Ensembl | Zoonomia |
+====+=====================+=========+============+=================+=========+==========+===========+============+
| 0 | LK_Cfam_Beagle_1.1 | 9615 | 2024/10/30 | GCA_044048985.1 | | | | |
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
| 1 | ASM4364393v1 | 9615 | 2024/10/24 | GCA_043643935.1 | | | | |
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
| 2 | UNSW_CanFamBas_1.2 | 9615 | 2021/02/23 | GCA_013276365.2 | | v | | |
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
| 3 | UMICH_Zoey_3.1 | 9615 | 2019/05/30 | GCF_005444595.1 | canFam5 | | v | v |
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
| 4 | ROS_Cfam_1.0 | 9615 | 2020/09/03 | GCF_014441545.1 | | v | v | |
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
| 5 | Yella_v2 | 9615 | 2023/09/07 | GCA_031165255.1 | | v | | |
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
| 6 | CA611_1.0 | 9615 | 2023/08/31 | GCA_031010295.1 | | v | | |
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
| 7 | OD_1.0 | 9615 | 2023/08/31 | GCA_031010635.1 | | v | | |
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
| 8 | BD_1.0 | 9615 | 2023/08/31 | GCA_031010765.1 | | v | | |
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
| 9 | Lak_Megaphage_Wal-2 | 9615 | 2024/06/07 | GCA_964164975.1 | | | | |
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
| 10 | Lak_Megaphage_Wal-1 | 9615 | 2024/06/07 | GCA_964162815.1 | | | | |
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
| 11 | UU_Cfam_GSD_1.0 | 9615 | 2020/03/10 | GCF_011100685.1 | canFam4 | v | v | v |
+----+---------------------+---------+------------+-----------------+---------+----------+-----------+------------+
...
# Check accessible data in databases
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
| | Assembly name | UCSC | RefSeq | GenArk | Ensembl | Zoonomia |
+====+=====================+=========+===============================+=======================+===========+==============+
| 0 | LK_Cfam_Beagle_1.1 | | | | | |
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
| 1 | ASM4364393v1 | | | | | |
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
| 2 | UNSW_CanFamBas_1.2 | | | agustus, xenoref | | |
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
| 3 | UMICH_Zoey_3.1 | canFam5 | gtf, gff, gnomon, cross, same | | ensembl | mouse, human |
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
| 4 | ROS_Cfam_1.0 | | gtf, gff, gnomon, cross, same | agustus, xenoref, ref | ensembl | |
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
| 5 | Yella_v2 | | | agustus, xenoref | | |
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
| 6 | CA611_1.0 | | | agustus, xenoref | | |
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
| 7 | OD_1.0 | | | agustus, xenoref | | |
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
| 8 | BD_1.0 | | | agustus, xenoref | | |
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
| 9 | Lak_Megaphage_Wal-2 | | | | | |
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
| 10 | Lak_Megaphage_Wal-1 | | | | | |
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
| 11 | UU_Cfam_GSD_1.0 | canFam4 | gtf, gff, gnomon, cross, same | agustus, xenoref, ref | ensembl | mouse, human |
+----+---------------------+---------+-------------------------------+-----------------------+-----------+--------------+
...
Search, download, and modify chromosome labels for various genome annotations, such as gaps and repeats
options:
-d types, --download types
Download annotation file.
gap : Genomic gaps - AGP defined (bigBed format)
sr : Simple tandem repeats by TRF (bigBed)
td : Tandem duplications (bigBed)
wm : Genomic intervals masked by WindowMasker + SDust (bigBed)
rmsk : Repeated elements annotated by RepeatMasker (bigBed)
cpg : CpG Islands - Islands < 300 bases are light green (bigBed)
gc : GC percent in 5-Base window (bigWig)
-c type, --chr_style type
Chromosome label style used in the download file (default: ensembl)
ensembl : 1, 2, X, MT & unknowns (GenBank IDs)
gencode : chr1, chr2, chrX, chrM & unknowns (GenBank IDs)
ucsc : chr1, chr2, chrX, chrM & unknowns (UCSC-specific IDs)
!! Limited use if UCSC IDs are not issued
raw : Uses raw file labels without modification
- NCBI GenBank: CM_* or other-form IDs
- NCBI RefSeq : NC_*, NW_* or other-form IDs
- GenArk : GenBank or RefSeq IDs
- Ensembl : Ensembl IDs
--recursive Download files regardless of their presence only if integrity check is not possible
# search usable and accessible data
gencube annotation canis_lupus_familiaris
gencube annotation GCF_011100685.1
# Download specific geneset file from a database
gencube annotation GCF_011100685.1 --download sr
gencube annotation GCF_011100685.1 --download td
gencube annotation GCF_011100685.1 --download rmsk
# Download multiple annotations from various databases
gencube annotation GCF_011100685.1 --download sr,td,rmsk,gcSearch and download sequence data of genesets
options:
-d types, --download types
Download "fasta" formatted sequence file
1. Nucleotide sequences:
refseq_rna : Accessioned RNA sequences annotated on the genome assembly
refseq_rna_genomic : RNA features based on the genome sequence
refseq_cds_genomic : CDS features based on the genome sequence
refseq_pseudo : Pseudogene and other gene regions without transcribed RNA or translated protein products
ensembl_cdna : Ensembl Rapid Release cDNA sequences of transcripts
ensembl_cds : Ensembl Rapid Release coding sequences (CDS)
ensembl_repeat : Ensembl repeat modeler sequences
2. Protein sequences:
refseq_pep : Accessioned protein sequences annotated on the genome assembly
refseq_pep_cds : CDS features translated into protein sequences
ensembl_pep : Ensembl Rapid Release protein sequences
--recursive Download files regardless of their presence only if integrity check is not possible
# search usable and accessible data
gencube sequence canis_lupus_familiaris
gencube sequence GCF_011100685.1
# Download specific geneset file from a database
$ gencube sequence GCF_011100685.1 --download refseq_rna
$ gencube sequence GCF_011100685.1 --download ensembl_cdna
$ gencube sequence GCF_011100685.1 --download ensembl_pep
# Download multiple genesets from various databases
$ gencube sequence GCF_011100685.1 --download refseq_rna,ensembl_cdna,refseq_pep,ensembl_pepSearch and download comparative genomics data, such as homology, and codon or protein alignment
options:
-d types, --download types
ensembl_homology : Homology data from Ensembl Rapid Release,
detailing gene orthology relationships across species
toga_homology : Homology data from TOGA, providing predictions of
orthologous genes based on genome alignments
toga_align_codon : Codon alignment data from TOGA, showing aligned codon
sequences between reference and query species
toga_align_protein : Protein alignment data from TOGA, detailing aligned
protein sequences between reference and query species
toga_inact_mut : List of inactivating mutations from TOGA, identifying
mutations that disrupt gene function
--recursive Download files regardless of their presence only if integrity check is not possible
# search usable and accessible data
gencube crossgenome canis_lupus_familiaris
gencube crossgenome GCF_011100685.1
# Download specific geneset file from a database
$ gencube crossgenome GCF_011100685.1 --download toga_homology
$ gencube crossgenome GCF_011100685.1 --download toga_align_codon
# Download multiple crossgenome data
$ gencube crossgenome GCF_011100685.1 --download toga_homology,toga_align_codonSearches for high-throughput sequencing data corresponding to user-specified keywords, retrieves the related sample metadata, and integrates it into experiment-level and study-level tables.
$ gencube seqmeta
usage: gencube seqmeta [-h] [-o string] [-st string] [-sr string] [-pl string] [-sl string] [-fi string] [-pr string] [-ly string] [-ac string] [-bp string] [-bs string]
[-as string] [-ti string] [-at string] [-pd range] [-md range] [-rl range] [-mb string] [-tw string] [-ex keywords] [-d] [-m]
[keywords ...]
Search, retrive, and integrate metadata of experimental sequencing data
positional arguments:
keywords Keywords to search for sequencing-based experimental data. You can provide various forms
Examples: liver, k562, cancer, breast_cancer, etc
Multiple keywords can be combined
Keywords separated by commas will combine their results
Keywords separated by spaces will intersect their results
Example: liver,lung cancer,tumor
options:
-h, --help show this help message and exit
-o string, --organism string
Scientific name or common name (as found in the NCBI Taxonomy Browser)
Example: homo_sapiens or human
-st string, --strategy string
Sequencing strategy:
wgs, wga, wxs, targeted_capture, synthetic_long_read, gbs, rad_seq, tn_seq, clone_end, amplicon
clone, rna_seq, mrna_seq, ncrna_seq, ribo_seq, rip_seq, mirna_seq, ssrna_seq, est, fl_cdna, atac_seq
dnase_hypersensitivity, faire_seq, chip_seq, chip, mre_seq, bisulfite_seq, mbd_seq, medip_seq, hi_c
chia_pet, tethered_chromatin_conformation_capture
-sr string, --source string
Source of the biological data:
genomic, genomic_single_cell, transcriptomic, transcriptomic_single_cell, metagenomic
metatranscriptomic, synthetic, viral_rna, other
-pl string, --platform string
Name of the sequencing platform:
abi_solid, bgiseq, capillary, complete_genomics, dnbseq, element, genapsys, genemind, helicos
illumina, ion_torrent, ls454, oxford_nanopore, pacbio_smrt, tapestri, ultima, vela_diagnostics
-sl string, --selection string
Library selection methodology:
5_methylcytidine_antibody, cage, cdna, cdna_oligo_dt, cdna_randompriming, chip, chip_seq, dnase
hmpr, hybrid_selection, inverse_rrna, mbd2_protein_methyl_cpg_binding_domain, mda, mf, mnase, msll
oligo_dt, other, padlock_probes_capture_method, pcr, polya, race, random, random_pcr
reduced_representation, repeat_fractionation, restriction_digest, rt_pcr, size_fractionation
unspecified
-fi string, --filter string
Option to find SRA records that are cross-referenced with other NCBI databases
(PubMed, PubMed Central (PMC), Nucleotide, Assembly, and others):
sra_all, sra_assembly, sra_bioproject, sra_bioproject_all, sra_biosample, sra_biosample_all, sra_gap
sra_gap_all, sra_gds, sra_genome, sra_nuccore, sra_nuccore_alignment, sra_nuccore_wgs, sra_omim
sra_pmc, sra_public, sra_pubmed, sra_taxonomy
-pr string, --properties string
Option to narrow search results by controlled-vocabulary library's annotations:
aligned_data, cloud_gs, cloud_s3, location_gs_us, location_s3_us_east1, location_s3_us_east_1
location_s3_us_west_2, filetype_10x_genomics_bam_file, filetype_ab1, filetype_activ_sars2_vcf
filetype_archive, filetype_archive/gzip, filetype_assembled_contigs
filetype_assembly/realign_summary, filetype_assembly_of_unidentified_reads, filetype_bai
filetype_bam, filetype_bam_header, filetype_basemodification, filetype_complete_genomics
filetype_crai, filetype_cram, filetype_fast5, filetype_fasta, filetype_fastq
filetype_geo_feature_count, filetype_helicos, filetype_illumina_native, filetype_nanopore
filetype_pacbio_base_modification_report, filetype_pacbio_metadata, filetype_pacbio_native
filetype_realign_to_de_novo_assembly, filetype_reference_fasta, filetype_run, filetype_run_realign
filetype_run_zq, filetype_sff, filetype_solid_native, filetype_source, filetype_sra_lite
filetype_sra_normalized, filetype_srf, filetype_tar_archive_of_complete_genomics_tree, filetype_tenx
filetype_vcf, filetype_vcf_index, filetype_vdbcache, filetype_vdbcache_zq, filetype_wgmlst_sig
filetype_wgmlst_signature, has_data, instrument_454_gs, instrument_454_gs_20, instrument_454_gs_flx
instrument_454_gs_flx_titanium, instrument_454_gs_junior, instrument_ab_310_genetic_analyzer
instrument_ab_3130_genetic_analyzer, instrument_ab_3130xl_genetic_analyzer
instrument_ab_3500_genetic_analyzer, instrument_ab_3500xl_genetic_analyzer
instrument_ab_3730_genetic_analyzer, instrument_ab_3730xl_genetic_analyzer
instrument_ab_5500_genetic_analyzer, instrument_ab_5500xl_genetic_analyzer
instrument_ab_5500xl_w_genetic_analysis_system, instrument_ab_solid_3_plus_system
instrument_ab_solid_4_system, instrument_ab_solid_4hq_system, instrument_ab_solid_pi_system
instrument_ab_solid_system, instrument_ab_solid_system_2_0, instrument_ab_solid_system_3_0
instrument_bgiseq_50, instrument_bgiseq_500, instrument_complete_genomics, instrument_dnbseq_g400
instrument_dnbseq_g400_fast, instrument_dnbseq_g50, instrument_dnbseq_t7, instrument_element_aviti
instrument_fastaseq_300, instrument_genexus, instrument_genolab_m, instrument_gridion
instrument_gs111, instrument_helicos_heliscope, instrument_hiseq_x_five, instrument_hiseq_x_ten
instrument_illumina_genome_analyzer, instrument_illumina_genome_analyzer_ii
instrument_illumina_genome_analyzer_iix, instrument_illumina_hiscansq
instrument_illumina_hiseq_1000, instrument_illumina_hiseq_1500, instrument_illumina_hiseq_2000
instrument_illumina_hiseq_2500, instrument_illumina_hiseq_3000, instrument_illumina_hiseq_4000
instrument_illumina_hiseq_x, instrument_illumina_hiseq_x_ten, instrument_illumina_iseq_100
instrument_illumina_miniseq, instrument_illumina_miseq, instrument_illumina_novaseq_6000
instrument_illumina_novaseq_x_plus, instrument_ion_genesudio_s5, instrument_ion_genesudio_s5_plus
instrument_ion_genesudio_s5_prime, instrument_ion_s5, instrument_ion_s5_xl
instrument_ion_torrent_genexus, instrument_ion_torrent_pgm, instrument_ion_torrent_proton
instrument_ion_torrent_s5, instrument_ion_torrent_s5_xl, instrument_mgiseq_2000rs, instrument_minion
instrument_nextseq_1000, instrument_nextseq_2000, instrument_nextseq_500, instrument_nextseq_550
instrument_onso, instrument_pacbio_rs, instrument_pacbio_rs_ii, instrument_promethion
instrument_revio, instrument_sentosa_sq301, instrument_sequel, instrument_sequel_ii
instrument_sequel_iie, instrument_tapestri, instrument_ug_100, instrument_unspecified
study_type_cancer_genomics, study_type_epigenetics, study_type_exome_sequencing
study_type_metagenomics, study_type_other, study_type_pooled_clone_sequencing
study_type_population_genomics, study_type_synthetic_genomics, study_type_transcriptome_analysis
study_type_transcriptome_sequencing, study_type_whole_genome_sequencing
-ly string, --layout string
Library layout of the sequencing data:
paired, single
-ac string, --access string
Data accessibility:
public, controlled
-bp string, --bioproject string
BioProject accession in the form of PRJNA#, PRJEB#, or PRJDB#
-bs string, --biosample string
BioSample accession in the form of SAMN#, SAMEA#, or SAMD#
-as string, --accession string
SRA/ENA/DDBJ accession
Study with accessions in the form of SRP#, ERP#, or DRP#
Sample with accessions in the form of SRS#, ERS#, or DRS#
Experiment with accessions in the form of SRX#, ERX#, or DRX#
Run with accessions in the form of SRR#, ERR#, or DRR#
-ti string, --title string
Descriptive name of the dataset
-at string, --author string
Researcher or group that submitted the data
Example: SON_KH
-pd range, --publication range
Publication Date
YYYY.MM.DD : YYYY.MM.DD format
Example: 2016, 2016.07, 2016.07.01, 2016.07:2023.02
-md range, --modification range
Modification Date
YYYY.MM.DD : YYYY.MM.DD format
Example: 2016, 2016.07, 2016.07.01, 2016.07:2023.02
-rl range, --readlength range
Length of the sequencing readsExample: 100 or 100:500
-mb string, --mbases string
Number of mega bases in the SRA Runs
-tw string, --textword string
General search term for finding datasets by specific words in metadata
-ex keywords, --exclude keywords
Exclude the results for the keywords used in this option
Example: cell_line,normal,crispr
-d, --detail Show the number of searched results for each option and keyword
-m, --metadata Save integrated metadata
-u, --url Save the file, including the URL address of the raw data (.fastq)
# Search for specific sequencing data for a specific species
$ gencube seqmeta --organism homo_sapiens --strategy rna_seq
$ gencube seqmeta --organism homo_sapiens --strategy chip_seq
$ gencube seqmeta --organism human --strategy chip_seq
# Search for cancer data for specific tissues
$ gencube seqmeta --organism human --strategy chip_seq liver,lung # liver OR lung
$ gencube seqmeta --organism human --strategy chip_seq cancer,tumor # cancer OR tumor
$ gencube seqmeta --organism human --strategy chip_seq liver,lung cancer,tumor # (liver OR lung) AND (cancer OR tumor)
# Exclude results containing specific keywords
$ gencube seqmeta --organism human --strategy chip_seq liver,lung cancer,tumor --exclude cell_line,crispr # cell_line OR crispr
# Use wild card (*) to search for a broader range of results
$ gencube seqmeta --organism human --strategy chip_seq liver,lung cancer*,tumor* # (liver OR lung) AND (cancer* OR tumor*)
# Use ^ for phrase (not word) search
$ gencube seqmeta --organism human --strategy chip_seq liver,lung cancer,tumor --exclude cell_line^,crispr # "cell line" OR crispr
# Search using accession
$ gencube seqmeta PRJNA838583
$ gencube seqmeta SRP375422
# or specifically
$ gencube seqmeta --bioproject PRJNA838583
$ gencube seqmeta --accession SRP375422
# Search using a custom query
$ gencube seqmeta '(((human[Organism]) AND ("chip seq"[Strategy])) AND ((liver OR lung) AND (cancer OR tumor)))'
# Output the number of search results for each option and keyword
$ gencube seqmeta --organism human --strategy chip_seq --exclude cell_line,crispr liver,lung cancer,tumor --detail
# Save the integrated metadata
$ gencube seqmeta --organism human --strategy chip_seq --exclude cell_line,crispr liver,lung cancer,tumor --metadata🔥 $ gencube seqmeta -o dog -st chip_seq --metadata --url
Experiment Type URL
SRX23379089 paired ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR277/078/SRR27712678/SRR27712678_1.fastq.gz
SRX23379089 paired ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR277/078/SRR27712678/SRR27712678_2.fastq.gz
SRX23379093 paired ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR277/074/SRR27712674/SRR27712674_1.fastq.gz
SRX23379093 paired ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR277/074/SRR27712674/SRR27712674_2.fastq.gz
SRX1726522 single ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR344/009/SRR3440079/SRR3440079.fastq.gz
SRX1559581 single ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR314/007/SRR3143427/SRR3143427.fastq.gz
SRX1559583 single ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR314/009/SRR3143429/SRR3143429.fastq.gz
...
🗂️ Displayed results
$ tree
├── gencube_output
│ ├── Canis_lupus_familiaris-CanFam3.1_chr_name.txt
│ ├── Canis_lupus_familiaris-Dog10K_Boxer_Tasha_chr_name.txt
│ ├── genome
│ │ ├── Canis_lupus_familiaris-CanFam3.1-refseq.sm.ens-id.fa.gz
│ │ └── Meta_genome_canfam3_complete-chromosome_241125_103030.txt
│ ├── geneset
│ │ ├── Canis_lupus_familiaris-CanFam3.1-refseq.ens-id.gtf.gz
│ │ └── Canis_lupus_familiaris-Dog10K_Boxer_Tasha-ensembl_ensembl.ens-id.gtf.gz
│ ├── annotation
│ │ ├── Canis_lupus_familiaris-CanFam3.1-genark.chrom.sizes.ens-id.txt
│ │ ├── Canis_lupus_familiaris-CanFam3.1-genark.gc5Base.ens-id.bw
│ │ └── Canis_lupus_familiaris-CanFam3.1-genark.simpleRepeat.ens-id.bb
│ └── sequence
│ │ ├── Canis_lupus_familiaris-CanFam3.1-refseq.protein.faa.gz
│ │ └── Canis_lupus_familiaris-CanFam3.1-refseq.rna.fna.gz
│ ├── crossgenome
│ │ ├── Canis_lupus_familiaris-UU_Cfam_GSD_1.0-ensembl_ensembl_homology.tsv.gz
│ │ ├── Canis_lupus_familiaris-UU_Cfam_GSD_1.0-toga_human_homology.tsv.gz
│ │ └── Canis_lupus_familiaris-UU_Cfam_GSD_1.0-toga_mouse_homology.tsv.gz
│ └── seqmeta
│ ├── Meta_seq_dog_241125_112002_experiment_n279.txt
│ ├── Meta_seq_dog_241125_112002_experiment_n279_urls.txt
│ └── Meta_seq_dog_241125_112002_study_n14.txt
└── gencube_raw_download
├── bedGraphToBigWig
├── bedToBigBed
├── bigBedToBed
├── bigWigToBedGraph
├── Canis_lupus_familiaris-CanFam3.1_assembly_report.txt
├── Canis_lupus_familiaris-CanFam3.1-genark.chrom.sizes.txt
├── Canis_lupus_familiaris-CanFam3.1-genark.gc5Base.bw
├── Canis_lupus_familiaris-CanFam3.1-genark.simpleRepeat.bb
├── Canis_lupus_familiaris-CanFam3.1-refseq.gtf.gz
├── Canis_lupus_familiaris-CanFam3.1-refseq.sm.fa.gz
├── Canis_lupus_familiaris-Dog10K_Boxer_Tasha_assembly_report.txt
└── Canis_lupus_familiaris-Dog10K_Boxer_Tasha-ensembl_ensembl.gtf.gz
🔥 Commands used to generate the above results
# Genome
$ gencube genome canfam3 --donwload
$ gencube genome canfam3 --metadata
# Gene set
$ gencube geneset canfam3 -d refseq_gtf
$ gencube geneset canfam3 -d ensembl_gtf
# Annotation
$ gencube annotation canfam3 -d sr
$ gencube annotation canfam3 -d gc
# Sequence
$ gencube sequence canfam3 -d refseq_rna
$ gencube sequence canfam3 -d refseq_pep
# Cross-genome
$ gencube crossgenome GCF_011100685.1 -d ensembl_homology
$ gencube crossgenome GCF_011100685.1 -d toga_homology
# Seqmeta
$ gencube seqmeta -o dog -st chip_seq --metadata --urlThis package was created with Cookiecutter and the audreyr/cookiecutter-pypackage project template.