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This notebook takes one 16S sample from an Illumina MiSeq run, runs it through Kraken 2 on Toolchest, and plots a rough sketch of the microbial composition of the sample.

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trytoolchest/getting-started-python-jupyter

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Getting started with Toolchest in Python

Intro

This repo has a Jupyter notebook with a basic microbiome analysis – from raw FASTQs to plots.

If you'd rather jump straight to a working version of this Jupyter notebook, head over to https://www.trytoolchest.com/getting-started-with-toolchest-python

Setup

Python dependencies

Quickest: from this directory, runpip install -r ./requirements.txt

Manual: install matplotlib, toolchest-client, and pandas

Toolchest key

Sign up for a free key, and replace the call to toolchest.set_key("YOUR_KEY_HERE") with your key.

Installing Jupyter

If you don't already have Toolchest installed locally, follow the Jupyter install guide.

Running

Run jupyter notebook microbiome_analysis.ipynb from inside the project to start a Jupyter notebook.

That should be it! You can advance through the notebook with the "Run" button, or however you like to run Jupyter notebooks.

About

This notebook takes one 16S sample from an Illumina MiSeq run, runs it through Kraken 2 on Toolchest, and plots a rough sketch of the microbial composition of the sample.

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