added s3-WGS

yanyang312 · Dec 11, 2022 · 6e9b993 · 6e9b993
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diff --git a/README.md b/README.md
@@ -63,6 +63,7 @@ Click the following links to view the methods. Notes:
   - [MALBAC](https://teichlab.github.io/scg_lib_structs/methods_html/MALBAC.html)
   - [scRRBS](https://teichlab.github.io/scg_lib_structs/methods_html/scRRBS.html)
   - [LIANTI](https://teichlab.github.io/scg_lib_structs/methods_html/LIANTI.html)
+  - [s3-WGS](https://teichlab.github.io/scg_lib_structs/methods_html/s3-WGS.html)
 
 - ### Multi-Omics
 

diff --git a/methods_html/s3-WGS.html b/methods_html/s3-WGS.html
@@ -0,0 +1,17 @@
+<!DOCTYPE html>
+<html lang="en">
+
+<head>
+<link rel="stylesheet" type="text/css" href="../style_related/page_format.css">
+<title>s3-WGS</title>
+</head>
+<body>
+
+<h1><a href="https://www.nature.com/articles/s41587-021-00962-z" target="_blank">s3-WGS</a></h1>
+
+<span style="font-size:1.1em;"><p>Due to semi-suppressive PCR (<a href="https://github.com/Teichlab/scg_lib_structs/issues/9#issuecomment-665438671" target="_blank">click here</a> to see a detailed explanation), only 50% of the tagmented material from the regular Tn5 are amplifiable. The <b>s3-WGS</b> approach introduced uracil bases in the Tn5 adapters and used uracil intolerant and tolerant DNA polymerases for the gap fill-in and PCR amplification, respectively. In this way, every Tn5 cutting event is amplifiable. The procedure is very similar to <a href="https://teichlab.github.io/scg_lib_structs/methods_html/s3-ATAC.html" target="_blank">s3-ATAC</a>, except that s3-WGS is perform on crosslinked and nucleosome-depleted nuclei.</p></span>
+
+<span style="font-size:1.1em;"><p>The library structure of <b>s3-WGS</b> is the same as <b>s3-ATAC</b>. <a href="https://teichlab.github.io/scg_lib_structs/methods_html/s3-ATAC.html" target="_blank">Click here</a> to see the detailed library generation steps.</p></span>
+
+</body>
+</html>