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added GIFs to demonstrate Cell counting
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sebi06 committed Mar 19, 2020
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23 changes: 23 additions & 0 deletions BioApps/README.md
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Expand Up @@ -23,3 +23,26 @@ The Image Analysis Settings (*.czias) can be used inside ZEN Blue or ZEN Core wi

Since the concept of **Open Application Development** (OAD) is about connecting the ZEN world with other valuable software tools, those example will also contain non-ZEN scripts etc.

## Counting Cells - the Classical Example

Those two short videos demonstrate how to do a simple cell count in ZEN blue abd display a heatmap. The [1st video](Count_Cells_Simple_Setup.gif) explains how to setup the IA analysis and the [2nd video](Run_Count_Cells_96wellplate_1GB_File_RealTime.gif) shows how is runs in Real Time (on a laptop).
Remark: The heatmap is shown at the end of the 2nd video.

![Setuo Image Analysis to Count Cells](Count_Cells_Simple_Setup.gif "Setuo Image Analysis to Count Cells")

![Setuo Image Analysis to Count Cells](Run_Count_Cells_96wellplate_1GB_File_RealTime.gif "Run Counting Cell IA Setting RealTime")

<p><img src="../Images/96well_heatmap.png" title="Heatmap from IA Analysis - Cell Count" width="700"></p>

Image Data:

* CZI file with 96 scenes from a typical wellplate sample carrier on a CellDiscoverer 7
* file size is rather small = approx. 1GB

The image analysis is quite straight forward and included the following steps:

* Smoothing using Median Filter
* Triangle Threshold
* Filter small particles
* Fill Holes
* Separate by Watershed
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